Generator

Part:BBa_K1859021:Experience

Designed by: Yusuke Banno   Group: iGEM15_Gifu   (2015-09-09)

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Applications of BBa_K1859021

[iGEM gifu 2015] .

We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane F shows this sample.


left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay

There was no fluorescence. We found that this generator doesn’t make functional long chain protein.

User Reviews

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